成人免费网站在线 I 亚洲经典视频在线观看 I 亚洲91精品 I 人人91 I 亚洲黄色短视频 I 国产成人无码av I 女性高潮视频 I 色女综合 I 性欧美69 I 欧美国产日韩在线观看成人 I 亚洲精品第五页 I 欧美另类videosbestsex日本 I 色综合激情网 I 冲田杏梨av I 男女操网站 I 日韩久久一区二区三区 I 91在线视频精品 I 北条麻妃在线一区 I 成色视频 I 亚洲熟女乱综合一区二区 I 欧亚一区二区三区 I 青青在线视频一区二区三区 I 精品国产一区二区亚洲人成毛片 I 成人久久18免费网站漫画 I 国产69精品久久久久777 I 亚洲色大网站www永久网站 I 国产欧美一区二区三区不卡高清 I 国产影片中文字幕 I 性感美女福利视频网站 I 精品久久久久久久久妇女 I 久久成人网站亚洲综合 I 天天操天天天 I 亚洲高清最新av网站 I 奇米影视亚洲狠狠色 I 丝袜美腿美女一区二区 I 欧美日韩一区二区三区在线观看免费 I 日本高清色www在线安全 I 久久人人做人人妻人人玩精品hd I 午夜少妇在线观看视频

CST Anti-GFP （D5.1） XP抗體

Specificity / Sensitivity

GFP （D5.1） XP^® Rabbit mAb detects GFP-tagged proteins exogenously expressed in cells.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to the amino terminus of GFP.

Western Blotting

Western blot analysis of extracts from HCC827 cells, untransfected or GFP-transfected, using GFP （D5.1） XP^® Rabbit mAb.

IHC-P （paraffin）

Immunohistochemical analysis of paraffin-embedded HCC827 cells, untransfected （left） or GFP-transfected （right）, using GFP （D5.1） XP^® Rabbit mAb.

Flow Cytometry

Flow cytometric analysis of HCC827 cells, untransfected （blue） or GFP-transfected （green）, using GFP （D5.1） XP^® Rabbit mAb.

IF-IC

Confocal immunofluorescent analysis of COS-7 cells expressing a GFP-tagged fusion protein （green） using GFP （D5.1） XP^® Rabbit mAb （red）. Blue pseudocolor = DRAQ5^® #4084 （fluorescent DNA dye）.

CST Anti-GFP （D5.1） XP抗體

Background

Epitope tags are useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation, and immunostaining techniques. Because of their small size, they are unlikely to affect the tagged protein's biochemical properties.

The green-fluorescent protein （GFP） functions as a bioluminescence energy transfer acceptor in the jellyfish Aequorea that maximally absorbs light at 395 nm and has an emission spectrum that peaks at 509 nm. GFP has become a very useful tool as a fusion protein that reports gene expression, traces cell lineage and defines subcellular protein localization （1）.

1. Miyawaki, A. et al. （2003） Curr Opin Chem Biol 7, 557-62.

Application References

• Matheny, R.W. and Nindl, B.C. （2011） Endocrinology 152, 1923-34. Applications: Western Blotting

Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!

Companion Products

• 2955 GFP （4B10） Mouse mAb
• 2372 β-Gal （14B7） Mouse mAb
• 2367 HA-Tag （6E2） Mouse mAb
• 2365 His-Tag Antibody
• 2276 Myc-Tag （9B11） Mouse mAb
• 7071 Phototope^®-HRP Western Blot Detection System, Anti-rabbit IgG, HRP-linked Antibody
• 7074 Anti-rabbit IgG, HRP-linked Antibody
• 7720 Prestained Protein Marker, Broad Range （Premixed Format）
• 7727 Biotinylated Protein Ladder Detection Pack
• 7003 20X LumiGLO^® Reagent and 20X Peroxide

For Research Use Only. Not For Use In Diagnostic Procedures.